On a night of records at the Rio Olympic trials in Adelaide, James Magnussen walked away empty handed.
Cameron McEvoy has become the first person to claim the national freestyle triple crown after taking 50m gold on Wednesday night.
And Cate Campbell set a new Australian record (23.93 seconds) to be fastest qualifier for Thursday night's 50m freestyle final.
It was just two tenths of a second outside the world record.
However, on a night the cream rose to the top Magnussen failed to snap up his last chance to secure an individual berth for Rio.
Magnussen finished third in the 50m freestyle, ensuring he must rely on being included in the 4x100m relay team to earn Rio selection.
Only a top two finish within the qualifying time secures an individual Rio nod.
The dual world champion earlier missed a spot in his pet event, the 100m freestyle by placing fourth in the final.
The Rio team will be confirmed on Thursday.
Magnussen refused to blame his shoulder that is recovering from 2015 surgery on his failure.
"You can never come into a meet like this with a negative mindset," he said.
"I've stayed positive the last nine months and I'm going to continue to try to stay positive otherwise I might go and bang my head against a couple of walls."
McEvoy clocked a personal best 21.44 seconds - the world's second fastest time of the year - to claim the 50m title ahead of Matt Abood (22.08) and Magnussen (22.12).
It marked the first time someone had won the national 50m, 100m and 200m crowns at the same meet since the one-lap event was added to the program in 1983.
McEvoy, 21, had earlier at the trials dead heated with Thomas Fraser-Holmes for the 200m gold and clocked the third fastest time ever to claim the 100m title.
"It's nice to be a part of history," McEvoy said.
"Personally I am more happy I can hit three decent times in those events."
In the women's 50m semis, Campbell backed up from her 100m success to again upstage world champion Bronte (24.27) to be fastest qualifier for the final.
Campbell said the pressure was now off earning Rio selection with her 100m win.
Asked what she would produce in the 50m final, Campbell said: "I think I want more of the same.
"I swim my best races when I am comfortable and controlled."
Meanwhile, Jess Ashwood (8:18.42) defended her 800m freestyle title, falling 0.01 short of her Australian record.