Reigning Paralympic and world champions spearhead Australia's powerful Paralympic cycling team announced 100 days out from the Games in Brazil.
The 13-strong team is headlined by seven-time Paralympian Kieran Modra and Sue Powell, who will chase a second consecutive gold medal.
The Rio team features two debutants; Alistair Donohoe and Kyle Bridgwood, who already has two world titles after less than two years of international experience.
Australia's Paralympics team chef de mission Kate McLoughlin said the 2016 group was one of the strongest Australia has produced, with nearly early every athlete having won either a Paralympic or world championship medal.
"I'm looking forward to seeing the team aim for a top three finish on the track, and to be amongst the top 10 in road cycling," said McLoughlin.
With five gold and three Paralympic bronze to his name, Modra came out of retirement for Rio.
South Australian Modra first competed at the Seoul 1988 Games in athletics. Transferring to swimming for the Barcelona 1992 Games, he made his Paralympic cycling debut in Atlanta 1996.
The tandem cyclist says he'll be chasing gold again in September.
"It has been an honour to take home a medal at nearly every games," he said.
"But what I'm most excited about is putting all my training together to seek a personal best."
Fellow reigning Paralympic champion Powell expects to turn up to Rio in the best form of her life.
"Being a Paralympian means personal excellence, regardless of what I can or can't do," she said.
The 49-year-old says she still gets goosebumps watching footage from her gold medal final from London.
Australia has won 34 gold, 24 silver and 26 bronze medal at the Paralympic Games since Atlanta 1996.
In London, Australia scored 14 medals, six of which were gold.
Australia's Paralympic cycling team:-
Kieran Modra (SA), Michael Gallagher (Vic), David Nicholas (Qld), Stuart Tripp (Vic), Kyle Bridgwood (Qld), Alistair Donohoe (Vic), Matt Formston (NSW), Jessica Gallagher (Vic), Carol Cooke (Vic), Simone Kennedy (NSW), Alexandra Lisney (NSW), Sue Powell (ACT), Amanda Reid (NSW), David Edwards (sighted pilot for Modra), Nick Yallouris (sighted pilot for Formston), Madison Janssen (sighted pilot for Gallagher).